Asparagus biflorus was first collected in 1938 by Reverend Jacob Gerstner, missionary to the Zululand area (1928-1942), at Ukuzi/Mkuzi Reserve, which was interpreted by Obermeyer and Immelman (1992) as referring to Mkuze Game Reserve, a protected area on the Maputaland coastal plain established in 1912. However, repeated surveys of the Mkuze reserve failed to relocate this subpopulation. Two other older collections are from the dry slopes of the Lebombo Mountains further north on the border between South Africa and Swaziland. Plants recently collected in the Lebombo Mountains vegetatively match this species, but were not in flower and more collections are needed to confirm the identification. In 2004, plants matching Asparagus biflorus were also collected in the Weenen district near the border with the Free State province, but in sparsely wooded montane grassland, a habitat completely different from either Mkuze or the Lebombo Mountains, and whether these plants are Asparagus biflorus or perhaps an undescribed taxon is not yet certain.

Obermeyer, A.A. and Immelman, K.L. 1992. Protasparagus. In: O.A. Leistner (ed). Flora of southern Africa 5 Part 3 Dracenaceae, Asparagaceae, Luzuriagaceae and Smilacaceae:11-70. National Botanical Institute, Pretoria.

Raimondo, D., von Staden, L., Foden, W., Victor, J.E., Helme, N.A., Turner, R.C., Kamundi, D.A. and Manyama, P.A. 2009. Red List of South African Plants. Strelitzia 25. South African National Biodiversity Institute, Pretoria.